Lesson 1 introduction to dna fingerprinting you are about to perform a procedure known as dna fingerprinting. The data obtained may allow you to determine if the samples of dna that you will be provided with are from the same individual or from different individuals.
Compare And Contrast Dna Rna Compare And Contrast Chemical And Physical Changes Contrast
The process of dna fingerprinting was developed by professor alec jeffreys at leicester university in 1984 as a form of genetic analysis.
Dna fingerprinting activity worksheet pdf. Dna fingerprinting after we isolate the dna and amplify it with pcr treat the dna with restriction enzymes ðcut dna at specific sequences. Gene activity is measured in this way and rna sampling is widely used to study development tissue specialization or the response of cells to various physiological stimuli. Dna restriction analysis is based on the following assumptions.
Fingerprints online pdf i use this worksheet after the first presentation. For this experiment it is necessary to review the structure of dna molecules. Make these on an 8 by 8 inch fingerprint identification card available from your local police department or your counselor.
The case of the crown jewels is an activity that simulates the dna fingerprinting process used by forensic scientists which relies on restriction analysis to analyze dna evidence from a fictional crime scene. Dna molecules can be identified by a difference in the sequence of bases. E the largest dna fragment on the gel belongs to the mother of couple b and baby 1 found at the top of the gel.
According to this information which sire was probably the father of this puppy. Dna fingerprinting b i o l o g y. Fingerprint guide pdf this one page handout includes examples for all of the ridge patterns and characteristics discussed in the fingerprinting lessons.
Because human dna is very similar to every other human s dna dna fingerprinting primarily focuses on the areas of the genetic code that vary. Dna fingerprinting relies on the fact that the dna code is universal for all living things and that there are differences between individuals within that code. C h a p t e r 9 purpose.
Using clear adhesive tape a pencil and plain paper record your own fingerprints or those of another person. A sample fingerprint card is included on the next page of this workbook c b. Dna fingerprinting worksheet 1.
The dna fingerprints were made from blood samples taken from a puppy and four possible sires of this puppy in an effort to determine the puppy s pedigree. To understand the basics of dna fingerprinting used in the canadian courts for crime convictions and paternity suits. An application solution lsm 6 3 6.
The picture shows a segment of. Student worksheet solutions additional activity. The smallest fragment on the gel belongs to the father or couple b found at the bottom of the gel.
Dna fingerprinting activity introduction.
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