Serial two fold and ten fold dilutions are commonly used to titer antibodies or prepare diluted analytes in the laboratory. Preform a sequence of dilutions to achieve a desired concentration.
Activity 1 1 5 Student Resource Sheet Serial
Stock solution test tubes pipettes beaker and distilled water.
Serial dilutions worksheet. Concentrations and dilutions answer key displaying top 8 worksheets found for this concept. Determine the amount plated the amount plated is the amount of dilution used to make the particular pour plate or spread plate. Dilutions worksheet solutions 1 if i have 340 ml of a 0 5 m nabr solution what will the concentration be if i add 560 ml more water to it.
Solution making and serial dilutions fall 2020 40 pts learning objectives. Dilutions worksheet solutions. 0 19 m the final volume is 900 ml set up the equation from that 2 if i dilute 250 ml of 0 10 m lithium acetate solution to a volume of 750 ml what will the concentration of this solution be.
Some of the worksheets for this concept are dilutions work dilutions work dilutions work name key dilutions work w 329 concentrations and dilutions molarity and serial dilutions teacher handout laboratory math ii solutions and dilutions calculationsforsolutionswork andkey. Complete this worksheet individually by following the directions. Take 4 test tubes label a b c and d.
The dilution factor in a serial dilution can be determined either for an individual test tube or can be calculated as a total dilution factor in the entire series. Serial dilution protocol pdf it is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. 0 19 m the final volume is 900 ml set up the equation from that 2 if i dilute 250 ml of 0 10 m lithium acetate solution to a volume of 750 ml what will the concentration of this solution be.
Prepare an aqueous solution of a specific concentration and volume. The dilution factor of each tube in a set. Previous dilution of 1 10 1st tube x 1 10 2nd tube total dilution of 1 100 10 2 1 10 2 step 3.
A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. 2 if water is added to 175 ml of a 0 45 m koh solution until the volume is 250 ml what. Dilutions worksheet w 329 everett community college student support services program 1 if 45 ml of water are added to 250 ml of a 0 75 m k 2 so 4 solution what will the molarity of the diluted solution be.
A series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration tubes containing your diluting liquid in exact quantities a tube containing an original. Properly identify the unknown variable in concentration problems. Each dilution will reduce the concentration of bacteria by a specific.
1 if i have 340 ml of a 0 5 m nabr solution what will the concentration be if i add 560 ml more water to it.
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